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  1. R

    Histogram Pixel Area Problem

    Awesome! I have everything figured out. So, for the histogram total pixel issue, all I have to do is make sure I uncache the histogram. Photoshop tells you when you need to with an exclamation point in the corner. If you don't, it may mess up the new histogram. I randomly figured this...
  2. R

    Histogram Pixel Area Problem

    Actually, the resolution should not matter because these pictures are taken from an electron microscope and it already displays a bar scale of the real life size in micrometers. All I have to do is go to Edit/Preferences/Units and Ruler and set the Ruler to pixels. From there, I can draw a...
  3. R

    Histogram Pixel Area Problem

    Okay, I'll let you know about the histogram calculation once I find out. As for the rest of the equation, does my equation make sense to convert the total pixels of the selection into centimeters? A=P/S^2 A=real area P=total pixel number in selection S=pixel/centimeter, measured from a bar...
  4. R

    Histogram Pixel Area Problem

    Yeah, I am only using one layer. I don't have that problem. Anyone else please?
  5. R

    Histogram Pixel Area Problem

    What do you mean by assuming I chose Selected Area? As of now, I just make the selection with the lasso tool and the histogram pixel # changes accordingly everytime I make a new selection. I'm not sure what you're referring to? Thanks again. Currently, the histogram reads 192,500 pixs for...
  6. R

    Histogram Pixel Area Problem

    Hello, For a research project, I am calculating the area of the cytosol of a cell by finding the total cell area and subtracting the total nuclear area. I have been using the lasso tool to select the boundaries and then the histogram to measure the total pixels in that selection area...
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