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Okay, I'll let you know about the histogram calculation once I find out.

As for the rest of the equation, does my equation make sense to convert the total pixels of the selection into centimeters?

A=P/S^2

A=real area

P=total pixel number in selection

S=pixel/centimeter, measured from a bar scale that came with the picture

I'm a bit confused on the S^2 part.  When you square that, woudn't it give you pixels^2/cm^2?  We want A=cm^2, but once we cross out all the units, we are left with A=pixel*cm^2 since P=pixel and not pixel^2.  People on another forum were claiming that equation works, but it looks flawed to me and the post is 10 years old so I can't ask on there ha.  Your thoughts?