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In designing packaging labels or related items, sometimes we have to include the barcode on the actual FA art (at the correct size needed) which are the ones used for prep in printing. Depending what barcode software was used, it may not have the controls for doing accurate sizing. Hence the need for proper handling in the layout design application.

A service printing bureau can also do this for you. But , to be on the safe side, I'd prefer doing it the way I want for fear the printers botch it up.